Ureaplasma test to detect its species and concentration by real-time PCR on woman’s genitourinary tract samples
Clinic role of ureaplasma is still being discussed.
On the one hand, researches show that, ureaplasma colonization of the lower part of the vagina may lead to complicated pregnancy such as preterm labour, underweight baby, and spontaneous labour. In men ureaplasma may cause urethritis and post-gonococcal complications.
On the other hand, ureaplasma is widespread among healthy people. Tests are done to distinguish infectious ureaplasma from normal. Up till recently, ureaplasma was considered as one species. Over time, scientists started distinguishing ureaplasmas. Now ureaplasmas found in human have two main types: ureaplasma urealyticum and ureaplasma parvum.
Ureaplasma urealyticum appeared to occur more often in non-gonococcal urethritis then ureaplasma parvum. Ureaplasma parvum is found more commonly in women and takes past in development of different pathologic processes.
Another marker characterizing ureaplasmas as to be able to induce a pathogenic process is its bacterial density or bacterial load. It is estimated by bacterial culture. It is expressed in colour changing units per 1 ml – CCU/ml.
Clinicians suppose that titer, or concentration, of 104 CCU/ml and higher means treatment for ureaplasma is to be started and 103 CCU/ml is considered to be normal.
The goal of the test is determine species and concentration respectively of U.urealyticum and U.parvum in women’s samples by real-time CPR. Samples are taken as scratches from cervical canal.
We suppose that dependence between bacterial density and manifestations of pathogenic processes is individual and requires for clinical supervision of patients. Therefore, we are convinced that, merely high ureplasma concentration cannot be considered as the only cause for prescription of antibacterial treatment. The test results acquired give ground to prolong investigations involving more people in contact with the person treated. Those under doctor’s supervision may both have genitourinary pathology and be healthy.